Dr. Kristaps Jaudzems

Senior Researcher, Group Leader

E-mail: kristaps.jaudzems@osi.lv
Tel.: (+371) 67014817

CV | Publications | Protein structures

Curriculum Vitae


Born February 5th, 1984 in Riga, Latvia

Education

03/2011 Ph. D. in Chemistry, Riga Technical University
Thesis: “NMR studies of protein structure, function and ligand interactions”. Supervisors: Prof. E. Liepinsh and prof. K. Wüthrich.
07/2008 M. Sc. in Chemical Technology, Riga Technical University
Thesis: “Protein NMR spectroscopy and spatial structure: examples of DnaI and cyclic Sortase A”. Supervisor: Prof. E. Liepinsh
07/2006 B. Sc. in Chemical Technology, Riga Technical University
Thesis: “Synthesis of selectively protected analogues of glucose-6-phosphate”. Supervisor: Prof. E. Bizdena

Research Positions and Training

09/2017-present Senior Researcher, Group Leader, Latvian Institute of Organic Synthesis
09/2015-08/2017 Marie Skłodowska-Curie Postdoctoral Fellow, Center for High Field NMR, Institute of Analytical Sciences, Lyon, France. Principal Investigator: Dr. Anne Lesage
11/2011-08/2015 Senior Researcher, Latvian Institute of Organic Synthesis
10-12/2014 Visiting Fellow, Australian National University, Canberra, Australia. Training in cell-free protein synthesis and paramagnetic NMR with Prof. Gottfried Otting
10-12/2012 Visiting Researcher, The Center for High-Field NMR, Lyon, France. Training in biomolecular solid-state NMR with Dr. Guido Pintacuda
06-09/2011 Postdoctoral Fellow (visiting), The Scripps Research Institute, La Jolla, CA, USA. Principal Investigator: Prof. Kurt Wüthrich
07/2010-05/2011 Research Assistant (Graduate Student), Latvian Institute of Organic Synthesis. Advisor: Prof. Edvards Liepinsh
09/2008-06/2010 External Graduate Student, The Scripps Research Institute, La Jolla, CA, USA. Advisor: Prof. Kurt Wüthrich
01/2007-08/2008 Research Assistant (Graduate Student), Latvian Institute of Organic Synthesis. Advisor: Prof. Edvards Liepinsh
05/2006-12/2006 Laboratory Assistant, Latvian Institute of Organic Synthesis
09/2002-05/2006 Laboratory Assistant, Riga Technical University

Teaching Experience

09/2017-present Associate Professor, Riga Technical University. Courses: Physical methods, NMR spectroscopy
02/2012-09/2017 Docent, Riga Technical University. Course: NMR spectroscopy for Master’s students
2013-2017 Lecturer, University of Latvia. Course: NMR spectroscopy for Master’s students

Language skills

Latvian (native), english and german (fluent), russian (basic)

Awards and Fellowships

10/2017 European Academy of Sciences and Arts promotion prize for young scientists
12/2013 Solomon Hiller award for BSc thesis supervisors (awarded by Riga Technical University and JSC OlainFarm)
09/2010-05/2011 European Social Fund fellowship
04/2009 Prof. Emīlija Gudriniece award to the best young scientist in chemistry
07/2006 JSC OlainFarm award for best BSc thesis

Publications

  1. Otikovs M., Andersson M., Jia Q., Nordling K., Meng Q., Andreas L.B., Pintacuda G., Johansson J., Rising A., Jaudzems K.. Degree of Biomimicry of Artificial Spider Silk Spinning Assessed by NMR Spectroscopy. Angew. Chem. Int. Ed. (2017) 56: 12571–12575. DOI
  2. Cala-De Paepe D., Stanek J., Jaudzems K., Tars K., Andreas L.B., Pintacuda G. Is protein deuteration beneficial for proton detected solid-state NMR at and above 100 kHz magic-angle spinning? Solid State Nucl. Magn. Reson. (2017) 87: 126–136. DOI
  3. Aleksis R., Oleskovs F., Jaudzems K., Pahnke J., Biverstål H. Structural studies of amyloid-β peptides: Unlocking the mechanism of aggregation and the associated toxicity. Biochimie (2017) 140: 176-192. DOI
  4. Kronqvist N., Sarr M., Lindqvist A., Nordling K., Otikovs M., Venturi L., Pioselli B., Purhonen P., Landreh M., Biverstål H., Toleikis Z., Sjoberg L., Robinson C.V., Pelizzi N., Jornvall H., Hebert H., Jaudzems K., Curstedt T., Rising A., Johansson J. Efficient protein production inspired by how spiders make silk. Nat. Commun. (2017) 8: 15504. DOI (Full text)
  5. Stanek J., Andreas L.B., Jaudzems K., Cala D., Lalli D., Bertarello A., Schubeis T., Akopjana I., Kotelovica S., Tars K., Pica A., Leone S., Picone D., Xu Z.-Q., Dixon N.E., Martinez D., Berbon M., El Mammeri N., Noubhani A., Saupe S., Habenstein B., Loquet A., Pintacuda G. NMR spectroscopic assignment of backbone and side-chain protons in fully protonated proteins: microcrystals, sedimented assemblies, and amyloid fibrils. Angew. Chem. Int. Ed. (2016) 55: 15504-15509. DOI
  6. Aleksis R., Jaudzems K., Muceniece R., Liepinsh E. Lunasin is a redox sensitive intrinsically disordered peptide with two transiently populated α-helical regions. Peptides (2016) 85: 56–62. DOI
  7. Recacha R., Jaudzems K., Akopjana I., Jirgensons A., Tars K. Crystal structure of Plasmodium falciparum proplasmepsin IV: the plasticity of proplasmepsins. Acta Cryst. (2016) F72: 659–666. DOI
  8. Shishovs M., Rumnieks J., Diebolder C., Jaudzems K., Andreas L.B., Stanek J., Kazaks A., Kotelovica S., Akopjana I., Pintacuda G., Koning R.I., Tars K. Structure of AP205 Coat Protein Reveals Circular Permutation in ssRNA Bacteriophages. J. Mol. Biol. (2016) 428: 4267–4279. DOI
  9. Andreas L.B., Jaudzems K., Stanek J., Lalli D., Bertarello A., Le Marchand T., Cala-De Paepe D., Kotelovica S., Akopjana I., Knott B., Wegner S., Engelke F., Lesage A., Emsley L., Tars K., Herrmann T., Pintacuda G. Structure of fully protonated proteins by proton-detected magic-angle spinning NMR. Proc. Natl. Acad. Sci. USA (2016) 113: 9187–9192. DOI
  10. Tjernberg L.O., Rising A., Johansson J., Jaudzems K. and Westermark P. Transmissible amyloid. J. Intern. Med. (2016) 280: 153–163. DOI
  11. Rasina D., Otikovs M., Leitans J., Recacha R., Borysov O.V., Kanepe-Lapsa I., Domraceva I., Pantelejevs T., Tars K., Blackman M.J., Jaudzems K., Jirgensons A. Fragment-Based Discovery of 2-Aminoquinazolin-4(3H)-ones As Novel Class Nonpeptidomimetic Inhibitors of the Plasmepsins I, II, and IV. J. Med. Chem. (2016) 59: 374–387. DOI
  12. Recacha R., Leitans J., Akopjana I., Aprupe L., Trapencieris P., Jaudzems K., Jirgensons A., Tars K. Structures of plasmepsin II from Plasmodium falciparum in complex with two hydroxyethylamine-based inhibitors. Acta Cryst. (2015) F71: 1531–1539. DOI
  13. Brangulis K., Jaudzems K., Petrovskis I., Akopjana I., Kazaks A., Tars K. Structural and functional analysis of BB0689 from Borrelia burgdorferi, a member of the bacterial CAP superfamily. J. Struct. Biol. (2015) 192: 320–330. DOI
  14. Otikovs M., Chen G., Nordling K., Landreh M., Meng Q., Jörnvall H., Kronqvist N., Rising A., Johansson J., Jaudzems K. Diversified Structural Basis of a Conserved Molecular Mechanism for pH-Dependent Dimerization in Spider Silk N-Terminal Domains. ChemBioChem (2015) 16: 1720–1724. DOI | Back Cover
  15. Chen W.N., Kuppan K.V., Lee M.D., Jaudzems K., Huber T., Otting G. O-tert-Butyltyrosine, an NMR tag for high-molecular weight systems and measurements of submicromolar ligand binding affinities. J. Am. Chem. Soc. (2015) 137: 4581–4586. DOI
  16. Andreas L.B., Le Marchand T., Jaudzems K., Pintacuda G. High-resolution proton-detected NMR of proteins at very fast MAS. J. Magn. Reson. (2015) 253: 36–49. DOI
  17. Jaudzems K., Pedrini B., Geralt M., Serrano P., Wüthrich K. J-UNIO protocol used for NMR structure determination of the 206-residue protein NP_346487.1 from Streptococcus pneumoniae TIGR4. J. Biomol. NMR (2015) 61: 65–72. DOI
  18. Zhulenkovs D., Rudevica Z., Jaudzems K., Turks M., Leonchiks A. Discovery and structure–activity relationship studies of irreversible benzisothiazolinone-based inhibitors against Staphylococcus aureus sortase A transpeptidase. Bioorg. Med. Chem. (2014) 22: 5988–6003. DOI
  19. Barbet-Massin E., Pell A.J., Retel J.S., Andreas L.B., Jaudzems K., Franks W.T., Nieuwkoop A.J., Hiller M., Higman V., Guerry P., Bertarello A., Knight M.J., Felletti M., Le Marchand T., Kotelovica S., Akopjana I., Tars K., Stoppini M., Bellotti V., Bolognesi M., Ricagno S., Chou J.J., Griffin R.G., Oschkinat H., Lesage A., Emsley L., Herrmann T., Pintacuda G. Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning. J. Am. Chem. Soc. (2014) 136: 12489–12497. DOI
  20. Andersson M., Chen G., Otikovs M., Landreh M., Nordling K., Kronqvist N., Westermark P., Jörnvall H., Knight S., Ridderstråle Y., Holm L., Meng Q., Jaudzems K., Chesler M., Johansson J., Rising A. Carbonic Anhydrase Generates CO2 and H+ That Drive Spider Silk Formation Via Opposite Effects on the Terminal Domains. PLoS Biol. (2014) 12: e1001921. DOI (Full text)
  21. Brangulis K., Petrovskis I., Kazaks A., Bogans J., Otikovs M., Jaudzems K., Ranka R., Tars K. Structural characterization of CspZ, a complement regulator factor H and FHL-1 binding protein from Borrelia burgdorferi. FEBS J. (2014) 281: 2613-2622. DOI
  22. Kronqvist N., Otikovs M., Chmyrov V., Chen G., Andersson M., Nordling K., Landreh M., Sarr M., Jörnvall H., Wennmalm S., Widengren J., Meng Q., Rising A., Otzen D., Knight S.D., Jaudzems K., Johansson J. Sequential pH-driven dimerization and stabilization of the N-terminal domain enables rapid spider silk formation. Nat. Commun. (2014) 5: 3254. DOI
  23. Jaudzems K., Tars K., Maurops G., Ivdra N., Otikovs M., Leitans J., Kanepe-Lapsa I., Domraceva I., Mutule I., Trapencieris P., Blackman M.J., Jirgensons A. Plasmepsin Inhibitory Activity and Structure-Guided Optimization of a Potent Hydroxyethylamine-Based Antimalarial Hit. ACS Med. Chem. Lett. (2014) 5: 373-377. DOI
  24. Zhulenkovs D., Jaudzems K., Zajakina A., Leonchiks A. Enzymatic activity of circular sortase A under denaturing conditions: An advanced tool for protein ligation. Biochem. Eng. J. (2014) 82: 200-209. DOI
  25. Aleksis R., Jaudzems K., Ivanova J., Žalubovskis R., Kalvinsh I., Liepinsh E. Reactivity of aziridine-2-carboxamide (Leakadine) with nucleophiles in aqueous solutions. Chem. Heterocycl. Comp. (2014) 49: 1589-1598. DOI
  26. Barbet-Massin E., Pell A.J., Jaudzems K., Franks W.T., Retel J.S., Kotelovica S., Akopjana I., Tars K., Emsley L., Oschkinat H., Lesage A., Pintacuda G. Out-and-back (13)C-(13)C scalar transfers in protein resonance assignment by proton-detected solid-state NMR under ultra-fast MAS. J. Biomol. NMR (2013) 56: 379-386. DOI
  27. Jaudzems K., Jia X., Yagi H., Zhulenkovs D., Graham B., Otting G., Liepinsh E. Structural Basis for 5′-End-Specific Recognition of Single-Stranded DNA by the R3H Domain from Human Sμbp-2. J. Mol. Biol. (2012) 424: 42-53. DOI
  28. Jaudzems K., Askarieh G., Landreh M., Nordling K., Hedhammar M., Jörnvall H., Rising A., Knight S.D., Johansson J. pH-Dependent Dimerization of Spider Silk N-Terminal Domain Requires Relocation of a Wedged Tryptophan Side Chain. J. Mol. Biol. (2012) 422: 477-487. DOI
  29. Johnson M.A., Jaudzems K., Wüthrich K. NMR structure of the SARS coronavirus nonstructural protein Nsp7 in solution at pH 6.5. J. Mol. Biol. (2010) 402: 619-628. DOI Full text
  30. Jaudzems K., Geralt M., Serrano P., Mohanty B., Horst R., Pedrini B., Elsliger M.-A., Wilson I.A., Wüthrich K. NMR structure of the protein NP_247299.1: comparison with the crystal structure. Acta Cryst. (2010) F66: 1367-1380. DOI (Full text)
  31. Mohanty B., Serrano P., Pedrini B., Jaudzems K., Geralt M., Horst R., Herrmann T., Elsliger M.-A., Wilson I.A., Wüthrich K. Comparison of NMR and crystal structures for the proteins TM1112 and TM1367. Acta Cryst. (2010) F66: 1381-1392. DOI (Full text)
  32. Serrano P., Pedrini B., Geralt M., Jaudzems K., Mohanty B., Horst R., Herrmann T., Elsliger M.-A., Wilson I.A., Wüthrich K. Comparison of NMR and crystal structures highlights conformational isomerism in protein active sites. Acta Cryst. (2010) F66: 1393-1405. DOI (Full text)
  33. Jaudzems K., Kuka J., Gutsaits A., Zinovjevs K., Kalvinsh I., Liepinsh E., Liepinsh E., Dambrova M. Inhibition of carnitine acetyltransferase by mildronate, a regulator of energy metabolism. J. Enz. Inhib. Med. Chem. (2009) 24: 1269-1275. DOI
  34. Loscha K.V., Jaudzems K., Ioannou C., Su X.-C., Hill F.R., Otting G., Dixon N.E., Liepinsh E. A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis DnaI helicase loader. Nucleic Acids Res. (2009) 37: 2395-2404. DOI (Full text)

Conference talks

  • Jaudzems K. Solution NMR conquers solids with fast MAS: de novo NMR structure determination of a 2.5 MDa capsid. Frontiers of NMR in Life Sciences, March 2017, Keystone, CO, USA.
  • Jaudzems K. Applications of solution and solid-state NMR in anti-infective drug discovery. EU H2020 ETN INTEGRATE Workshop, February 2017, Riga, Latvia.
  • Jaudzems K. Structure determination of non-diffracting protein assemblies by solid state NMR. 7th Annual BioStruct Conference, August 2016, Jægtvolden, Norway.
  • Jaudzems K. De novo 100 kHz MAS structure determination of a viral nucleocapsid and insights from high field DNP. EUROMAR 2016, July 2016, Aarhus, Denmark.
  • Jaudzems K. De novo 100 kHz MAS structure determination of a viral nucleocapsid and insights from high field DNP. 1ère Journée Grand-Sud de RMN, May 2016, Montpellier, France.
  • Jaudzems K. Fragment based discovery of 2-aminoquinazolin-4-ones as novel anti-malarial agents targeting plasmepsins. Drug Discovery Conference, August 2015, Riga, Latvia.
  • Jaudzems K. Spider silk proteins in soluble and amyloid states. Nobel Minisymposium “Amyloid – a multifaceted player in human health and disease”, June 2015, Stockholm, Sweden.
  • Jaudzems K. KMR metodes jaunu zāļu pētniecībā. 73rd Conference of University of Latvia, February 2015, Riga, Latvia.
  • Jaudzems K. Fragment-based drug discovery for malaria. Advanced anti-infective drug discovery and design, May 2014, Riga, Latvia.
  • Jaudzems K. Mechanism of spider silk formation studied by NMR. 4th Bio-NMR Annual User Meeting, May 2014, Warsaw, Poland.
  • Jaudzems K. 1H-based backbone assignment strategy using CARA. pNMR Applied Training Course “Solid State NMR of Materials and Proteins”, September 2013, Lyon, France.
  • Jaudzems K. Fragment based drug discovery of novel plasmepsin II inhibitors using NMR. 3rd Bio-NMR Annual User Meeting, June 2013, Budapest, Hungary.
  • Jaudzems K. Protein structure and function depends on water environment conditions. Estonian Magnet Week, October 2012, Tallinn, Estonia.
  • Jaudzems K. Structural studies of spider silk N-terminal domain: insights into the mechanism of fiber formation. 2nd EAST-NMR Young Investigators Meeting, May 2012, Bratislava, Slovakia.
  • Jaudzems K. Kodolu magnētiskās rezonanses pētījumi jaunu zāļu vielu atklāšanai. 3rd World Congress of Latvian Scientists, October 2011, Riga, Latvia.
  • Jaudzems K. NMR studies of nucleotide binding to the R3H domain. EAST-NMR regional meeting “NMR and complementary tools for chemistry and biology research”, June 2011, Riga, Latvia.
  • Jaudzems K. Nucleotide binding to the R3H domain studied by NMR. 1st EAST-NMR Young Investigators Meeting, September 2010, Istanbul, Turkey.
  • Jaudzems K. Automation in the NMR structure determination of a 206-residue protein. JCSG 8th Annual Meeting, April 2009, La Jolla, CA, USA.

Patents

  1. Kinena L., Ozola V., Suna E., Leitis G., Jirgensons A., Jaudzems K., Kanepe-Lapsa I., Domracheva I. Substituted aminoalkylazoles as malarial aspartic protease inhibitors. WO2017069601.
  2. Pelšs J., Domračeva I., Žalubovskis R., Jaudzems K. Derivatives of 2,6-diaza-bicyclo[2.2.2]octane-3,5-ditione as potential anti-cancer agents. LV15160.
  3. Jirgensons A., Domraceva I., Kanepe-Lapsa I., Rasina D., Jaudzems K., Otikovs M. Novel substituted 2-aminoquinazolin-4(3H)-one derivatives as malarial aspartic protease inhibitors. WO2015063544.
  4. Zhulenkovs D., Rudevica Z., Leonchiks A., Jaudzems K., Zicane D., Turks M. N-(3-hydroxy-5,7-dimethyladamantan-1-yl)-2-(3-oxobenzo[d]isothiazol-2(3h)-yl)acetamide as a novel inhibitor for staphylococcus aureus sortase a. EP2875816.
  5. Zhulenkovs D., Rudevica Z., Leonchiks A., Jaudzems K., Zicane D., Turks M. N’-(2-(3-oxobenzo[d]isothiazol-2(3h)-yl)acetyl)adamantane-1-carbohydrazide as a novel inhibitor for staphylococcus aureus sortase A. EP2875815.

Protein structures

17. Solid-state MAS NMR structure of Acinetobacter phage 205 (AP205) coat protein in assembled capsid particles
16. NMR structure of N-terminal domain from A. ventricosus minor ampullate spidroin (MiSp) at pH 5.5
15. NMR structure of N-terminal domain from A. ventricosus minor ampullate spidroin (MiSp) at pH 7.2
14. Solution structure of sortase A from S. aureus in complex with benzo[d]isothiazol-3-one based inhibitor
13. NMR structure of a putative phosphoglycolate phosphatase (NP_346487.1) from Streptococcus pneumoniae TIGR4
12. NMR structure of C-terminal domain from A. ventricosus minor ampullate spidroin (MiSp)
11. NMR structure of major ampullate spidroin 1 N-terminal domain at pH 5.5
10. Solution structure of the R3H domain from human Smubp-2 in complex with 2′-deoxyguanosine-5′-monophosphate
9. NMR structure of major ampullate spidroin 1 N-terminal domain at pH 7.2
8. NMR structure of a monomeric mutant (A72R) of major ampullate spidroin 1 N-terminal domain
7. NMR structure of the protein YP_557733.1 from Burkholderia xenovorans
6. NMR structure of the protein NP_415897.1
5. NMR Structure of the SARS Coronavirus Nonstructural Protein Nsp7 in Solution at pH 6.5
4. NMR structure of heavy metal binding protein TM0320 from Thermotoga maritima
3. Solution NMR structure of the thermotoga maritima protein TM0855 a putative ribosome binding factor A
2. NMR STRUCTURE OF A PUTATIVE DINITROGENASE (MJ0327) FROM METHANOCOCCUS JANNASCHII
1. N-terminal domain of the Bacillus subtilis helicase-loading protein DnaI

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